A Novel “Inocap” Method for Homogeneous Catheter Hubs Bacterial Inoculation Using a Combination of Male Luer Caps and O-rings.

William W. Tang, Benyarat Wiriyanithinon, Nathan S. Kim, Chanond Sophonpanich, Tanant Waritanant

Abstract


Catheter-related bloodstream infections (CRBSIs) pose significant health risks and financial consequences for healthcare systems worldwide. In order to develop novel antiseptic technologies as alternative prophylactics for CRBSIs, a consistent and reproducible bacterial inoculation method is required for testing the technologies’ antiseptic effects. This study developed and investigated a new “Inocap” model for standardized bacteria inoculation on to surfaces of catheter hubs that is exposed and highly susceptible to contamination in healthcare settings. The results showed a significantly smaller variance in the number of bacteria inoculated onto the catheter hubs by the new Inocap method than the traditional dip method (p ≈ 0.02) while producing no significant difference in the mean value (p > 0.25), indicating that this new Inocap method could work as a more precise inoculation model for future disinfection studies.


Address Correspondence to author:
2301/2 New Petchburi Rd. Bangkapi, Huaikwang, Bangkok Thailand 10310 email: tanant.w@cathecare.com

Keywords:
Bacteria inoculation model, disinfection, catheter related bloodstream infections, intraluminal infection, catheter hubs, contamination

Address Correspondence to author:
2301/2 New Petchburi Rd. Bangkapi, Huaikwang, Bangkok Thailand 10310 email: tanant.w@cathecare.com

Received: June 30, 201
Revision received: July 3, 2018
Accepted after revision: July 10, 2018
BKK Med J 2018;14(2): 28-33.

DOI: 10.31524/bkkmedj.2018.09.006

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